Learn Variant Calling of NGS data using pipeline development
Hands on Bioinformatics Pipeline Implementation for Calling Variant’s on Linux OS and WSL on windows
Description
Demystifying Genomic Data Analysis: Unlocking the Power of Bioinformatics
Are you a student at the intersection of molecular genetics and bioinformatics, seeking to unravel the mysteries that lie ahead? Do you recognize the growing demand for computational skills in life sciences research and wonder how they apply to diverse biological data? Are you ready to embrace the Linux operating system as a tool to tackle multi-omics problems and witness the evolution of bioinformatics and computational biology in addressing modern biological challenges?
The time has come for biologists to extend their reach beyond wet labs, as the boundaries between wet and dry labs blur. Join us on a transformative journey, empowering yourself with the data science tools of bioinformatics for analysis, visualization, and inferring crucial insights from large-scale genomic datasets. This course serves as the perfect stepping stone, facilitating your transition from wet to dry labs and equipping you to analyze whole-genome NGS data for variant calling, an essential process that reveals the intricate differences within genomes.
Variant calling plays a pivotal role in establishing a comprehensive foundation for subsequent functional gene fine mapping. By conducting whole-genome or whole-exome sequencing and generating FASTQ files, you lay the groundwork. Aligning these sequences to a reference genome, you create BAM or CRAM files. Finally, identifying where the aligned reads deviate from the reference genome and recording these differences in a VCF file completes the variant calling process.
In this course, we provide comprehensive training on variant calling and guide you through the steps involved in processing and analyzing genomic data. You'll learn the fundamental principles of variant calling, enabling you to explore the entire genome base by base and extract a wealth of molecular markers.
Through practical exercises and real-world examples, you'll develop the skills necessary to carry out variant calling with speed, accuracy, and efficiency. As you gain proficiency in this critical process, you'll lay a solid foundation for subsequent analyses and fine mapping of functional genes.
Join us in this transformative course and become proficient in analyzing genomic data for variant calling. Embrace the power of bioinformatics tools and make meaningful contributions to the rapidly evolving field of modern biology.
Enroll now and unlock the potential of your big genomic datasets, as you embark on a journey towards deeper insights and impactful discoveries.
What You Will Learn!
- understanding bioinformatics pipelines
- what are genetic variants
- Understand the data set
- Explain how a FASTQ file encodes per-base quality scores.
- Interpret a FastQC plot summarizing per-base quality across all reads.
- Clean FASTQ reads using Trimmommatic.
- Select and set multiple options for command-line bioinformatics tools.
- Understand the steps involved in variant calling.
- Describe the types of data formats encountered during variant calling.
- Use command line tools to perform variant calling.
- Quickly Learn the about Linux Command Line
- Gain a Complete Understanding of CLI and GUI OS
- Operate a Linux OS like Ubuntu on Microsoft Windows
- Learn how to Manage Open-Source Software with ubuntu Terminal
Who Should Attend!
- People generally interested in new research methodologies and would like to try them themselves!
- Beginner Bioinformaticians looking to understand the process of NGS and Variants