Master Primer Designing & Polymerase Chain Reaction (PCR)

Learn Primer Designing and In-silico Polymerase Chain Reaction(PCR) amplification using Bioinformatics Tools.

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Description

In this course you will learn:

  • The basic concepts of the standard polymerase chain reaction (PCR) technique.

  • The criteria required to design a DNA primer for PCR.

  • The online programs we usually use to design a DNA primer.


Polymerase chain reaction (PCR)

You will learn in this section:

  • Steps involved in the PCR (PCR cycling): denaturation, annealing, extension

  • Components of the PCR reaction: DNA template, DNA polymerase, dNTPs, forward primer and reverse primer.

  • PCR amplification program.

  • Exponential amplification.

  • The size difference between the PCR amplification products of the first, second, and third cycle.

Criteria for PCR primer design

You will learn in this section a detailed explanation of the PCR primer design criteria and how they affect the primer sensitivity and stability including; primer length, primer melting temperature, primer annealing temperature, GC% of the primer, GC-clamp, cross homology and primer secondary structure.

Tools and methods

In this section you will learn how to:

  • Retrieve a Gene Sequence form NCBI, and Determine the Exact Location for Each Exon on the Chromosome Using Graphics.

  • Compare Different mRNA Transcripts and Select One to Evaluate a Gene Expression in Novel Cells.

  • Understand Primer3 Setting.

  • Calculate Primer Self-Complementarity Score.

  • Check for Primer Cross Homology Using BLAT.

  • In_silico PCR, How to confirm PCR amplification

What You Will Learn!

  • You will learn Brief Introduction to PCR Including (Principle, Procedure, Stages, and Optimization)
  • You will learn Applications of PCR
  • You will learn Advantages & Limitations of PCR
  • You will learn Variations and History of PCR
  • You will learn Primer Designing | Primer Specification or Primer Requirements
  • You will learn Primer Designing (Manually) by ClustalW & Oligocalculator
  • You will learn about Primer3 tool | Automated method for Primer Designing
  • You will learning about Primer validation by BLAST
  • You will learn In_silico PCR | How to confirm PCR amplifiacion

Who Should Attend!

  • Bioinformaticians & Biotechnologists are Encouraged to take this course.
  • Entry Level Primer Designer
  • Researchers are also encouraged to take this Course
  • Genetic laboratory professionals who work on PCR workbench to gain additional knowledge
  • Anyone who want to learn something New