Molecular biology

Introduction to world of Molecular biology

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Description


Introduction to the course

Provides information on

  • Brief introduction on Tutor

  • Benefits from the course

  • Topics/Structure of the course

  • Who can enroll to the course


UNIT 1 : Replication 

  • Central Dogma of biology

  • Replication of DNA

  • Semiconservative replication

  • Complimentary base pairing

  • Supercoil and DNA topoisomerase

  • Replication fork

  • DNA synthesis catalysed by DNA Polymerase

  • DNA Damage and Repair

  • Nucleotide Excision Repair

  • Base Excision Repair

  • Mismatch Repair system


UNIT 2:  Transcription

  • Central Dogma of Life

  • what is Transcription

  • Mechanism Of Transcription

  • In Prokaryotes

    • Initiation

    • elongation

    • Termination

  • In Eukaryotes

    • Initiation is initiated with help of promotor region

    • TAAT Box and Pribnow Box

    • Elongation

    • Enhancers and Silencers

    • Termination

  • Post Transcriptional Modification

    • Addition of Cap and Poly A tail

    • Splicing Of mRNA 

    • mRNA Editing

    • Inhibitors of Transcription

    • Reverse Transcriptase and RNA Degradation.


UNIT 3 : Translation

  • Genetic Code

  • Features Of Genetic Code

  • Codon - Anticodon Pairing

  • wobble Hypothesis

  • Link Between Amino Acid And Nucleic Acid

    Translation In Prokaryotes   

  • Initiation

  • Elongation

  • Termination

  • How much Energy is consumed during the Process

    Translation  in Eukaryotes

  • Initiation with Pre Initiation complex

  • Association of Ribosomal Subunit

  • Elongation

  • Termination using Termination Codons

  • Release Factors

    Post Translational Modification

  • Trimming

  • Structural Reorganisation

  • Modification at N and  C Terminal Ends

  • Covalent Modification

  • How much Of energy is consumed during the process of Translation


    Unit 4:  Regulation of Gene Expression

  • Enhancers and silencers

  • House keeping and invisible genes

  • One cistron -one subunit concept

  • DNA organisation and Gene expression

  • Covalent Modification of Histones control Gene expression

  • Regulation of Gene Expression in Prokaryotes :

  • Lac operon

  • structure of Lac Operon

  • Tryptophan regulation in Eukaryotes

  • Structure of Tryptophan Operon with structural gene


What You Will Learn!

  • Definition of Replication, process of replication in prokaryotes and eukaryotes
  • DNA damage and repair system
  • Definition of transcription, process of transcription in prokaryotes and eukaryotes
  • Post transcriptional modification and drugs which inhibit transcription
  • Translation, process of translation in both prokaryotes and eukaryotes.
  • Post translational modification
  • Regulation of Gene expression, lacoperon, tryoperon

Who Should Attend!

  • Students who are in graduation level in the field of biology, molecular biology and Biotechnology