Molecular Biology and biotechnology principles

Recombinant DNA Technology , Polymerase Chain Reaction ,Electrophoresis , Chromatography, Blotting technique and ELISA

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Description

In this course students will learn about molecular biology and biotechnology principles.


1.  Unit 1

Topics covered in : Lecture 1 , 2 and 3

  • Recombinant DNA technology

  • Tools used in Recombinant DNA technology

  • Isolation of vector gene

  • Isolation of desired gene

  • Methods to transfer foreign gene into bacterial cell

  • Discuss about Restriction enzymes

  • And about ligases

  • Vectors like cosmid, plasmid

  • Production of insulin gene

  • Applications of recombinant DNA technology


2. Unit 2

Topics covered in Lecture 4

Molecular biology Techniques

Southern blotting technique used for DNA samples

Northern blotting used for RNA samples 


3. Unit 3

Topics covered in Lecture 5

  • Hybridisation technique

  • Polymerase chain reaction used to amplify the DNA in the sample

  • Radio immuno Assay - Antigen and antibody specific

  • Electrophoresis

  • zone electrophoresis

  • Gel electrophoresis

  • Polyacrylamide gel electrophoresis.

  • Paper electrophoresis.

  • Principle of electrophoresis.

  • Used to separate the DNA depending on the size and charge on the molecule.

  • Gel electrophoresis - is used to separate DNA or RNA.

  • SDS page used to separate proteins and amino acids


4. Unit 4

Topics covered in Lecture 6 and 7

  • chromatography

  • column chromatography

  • Affinity chromatography.- based on affinity towards ligand and protein .

  • Ion exchange chromatography.

  • Cat-ionic exchanger and anionic exchanger

  • Separation is based on ions present in the protein sample

  • Gas liquid chromatography. Gas acts as a mobile phase and liquid acts as a stationary phase .gases like inert gases are used and liquid of high boiling point is used in gas liquid chromatography

  • Paper chromatography . Paper is used and reagent are used to identify the compound present in the sample

  • principle of chromatography

  • separation of molecules based on size, charge and molecular weight

  • Separation of macro molecules based on antigen and antibody interaction

  • Enzyme and ligand interaction as there is a specific interaction between the antigen and antibody interaction


    Summary of the course


Add-on

  • Course would be added/enhanced based on Q&A form students in to form of additional information




What You Will Learn!

  • Recombinant DNA Technology or Cloning
  • Polymerase Chain Reaction
  • Hybridisation and Blotting Techniques
  • Principles of Chromatography and Electrophoresis
  • Principles of electrophoresis

Who Should Attend!

  • Students who are in the field of Molecular Biology ,Biotechnology and students interested in Research Field