Description
Part 1:
what is PCR?
who invented PCR?
How does PCR works?
PCR chemical ingredients
PCR Diagram
Steps:
1 - Denaturation
2- Annealing
3-Extension
Part 2:
Types of PCR
Quantitative
Real time
Reverse transcription
Part 3:
Multiplex
Nested
High -fidelity
fast
heart start
GC rich
Long range
AP PCR
Part 4:
application of PCR
clinical diagnosis, prenatal diagnosis, retro-viral, bacterial infection, cancer diagnosis, sex determination embryos and forensic medicine
Standard ingredients in the mixture are:
·the DNA segment of interest
·specific primers
·heat-resistant DNA polymerase enzyme
·the four different types of DNA nucleotides
·the salts needed to create a suitable environment for the enzyme to act.
Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.
Real-time PCR is the technique of collecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step. This is achieved using a variety of different fluorescent chemistries that correlate PCR product concentration to fluorescence intensity
Reverse transcription polymerase chain reaction is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction. It is primarily used to MEA
sure the amount of a specific RNA.
Direct detection of microorganisms in patient specimens
Identification of microorganisms grown in culture
Detection of antimicrobial resistance
Investigation of strain relatedness of a pathogen of interest
Genetic fingerprinting (forensic application/paternity testing)
Detection of mutation ( investigation of genetic diseases)
you can have maximum details in course video
What You Will Learn!
- what is PCR?
- PCR types?
- Steps of PCR
- Applications
Who Should Attend!
- Bsc Bio-technoligy
- BIO- chemistry
- Microbiology
- B pharmacy
- M pharmacy
- Msc Biotechnology
- Msc Mircobiology
- Msc Biochemistry
- Medical students